CSME Conference Proceedings (May 27-30, 2018)

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Open Access
Controlled Deflection Of Diamagnetic Biocompatible Aqueous Droplets
(May-18) Kieda, Jennifer; Tsai, Scott; Buryk, Stephanie
We present a microfluidic platform that is capable of controlled diamagnetic droplet displacement. In this method, monodisperse aqueous droplets are produced in a continuous phase of hydrophobic ferrofluid. Both phases are exposed to magnetic field. Precise deflection of the aqueous droplets is then achieved in a single step by adjusting the flowrate of the disperse phase.
Open Access
Investigation Of Epithelial-To-Mesenchymal Transition Through Microcontact Printing
(CSME-SCGM, May-18) Inayat, Huma; Tsai, Scott; Kapus, Andras
We have used microcontact printing as a means of simulating injury or wounds in a healthy monolayer of LLC PK1 cells to study the expression of Epithelial-to-Mesenchymal Transition (EMT) signature proteins. Cells were patterned on PDMS substrates using stamps that were prepared in the cleanroom using conventional soft lithography techniques. Patterned cells were exposed to an external stimulus of Transformation Growth Factor β (TGFβ) and analyzed further for the presence and upregulation of EMT signature proteins that are indicative of the progression of EMT.