Analyzing Vitamin D3 Supplementation and Deficiency in the Spinal Cord of Transgenic G93A Mouse Model of Amyotrophic Lateral Sclerosis

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dc.contributor.advisorHamadeh, Mazen J.
dc.creatorMoghimi, Elnaz
dc.date.accessioned2015-08-28T15:27:27Z
dc.date.available2015-08-28T15:27:27Z
dc.date.copyright2015-03-03
dc.date.issued2015-08-28
dc.date.updated2015-08-28T15:27:26Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractVitamin D3 (D3) may impact ALS, a motoneurodegenerative disease. The study analyzed D3 supplementation at 50x the adequate intake (AI) and restriction at 2.5% the AI in the spinal cord of transgenic G93A, a mouse model of ALS. At 25 d, mice were provided food ad libitum with adequate (AI;1 IU D3/g feed), high (HiD; 50 IU D3/g feed) or deficient (DEF; 0.025 IU D3/g feed) D3. At 113 d, the spinal cords underwent protein analysis. HiD females exhibited D3 toxicity, evidenced by increased oxidative damage and apoptosis and lower antioxidant capacity vs. AI. HiD males exhibited lower oxidative damage, inflammation, apoptosis and neuron damage vs. AI. DEF females exhibited higher inflammation and a compensatory increase in GPx1 vs. AI. DEF males exhibited higher lipid peroxidation and lower antioxidant capacity vs. AI. In G93A mice, non-toxic doses of D3 attenuate disease pathophysiology, whereas deficiency worsens it in a sex-specific manner.
dc.identifier.urihttp://hdl.handle.net/10315/30029
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectNutrition
dc.subjectPhysiology
dc.subjectHealth sciences
dc.subject.keywordsVitamin D
dc.subject.keywordsHypovitaminosis D
dc.subject.keywordsHypervitaminosis D
dc.subject.keywordsAmyotrophic lateral sclerosis
dc.subject.keywordsG93A mouse
dc.subject.keywordsSpinal cord
dc.subject.keywordsMotor neurons
dc.subject.keywordsNeuroprotection
dc.subject.keywordsOxidative stress
dc.subject.keywordsInflammation
dc.subject.keywordsAntioxidant capacity
dc.subject.keywordsApoptosis
dc.subject.keywordsNeurotrophic factors
dc.subject.keywordsNeuron count
dc.subject.keywordsNeuron damage
dc.titleAnalyzing Vitamin D3 Supplementation and Deficiency in the Spinal Cord of Transgenic G93A Mouse Model of Amyotrophic Lateral Sclerosis
dc.typeElectronic Thesis or Dissertationen_US

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