Methods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg types

dc.contributor.authorForer, Arthur
dc.contributor.authorGauthier, Kimberley
dc.contributor.authorFerraro-Gideon, Jessica
dc.contributor.authorHoang, Carina
dc.date.accessioned2021-02-22T15:26:17Z
dc.date.available2021-02-22T15:26:17Z
dc.date.issued2013-10
dc.description.abstractMesostoma ehrenbergii spermatocytes are uniquely useful to study various aspects of cell division. Their chromosomes are large in size and few in number, with only 3 bivalent and 4 univalent chromosomes. During prometaphase, bipolar bivalents oscillate regularly to and from the poles for 1-2 hours. The univalents remain at the poles but occasionally move from one pole to the other. In addition, a precocious cleavage furrow forms during prometaphase and remains partially constricted until anaphase. Attempts to rear these animals indefinitely in laboratory conditions, however, have been mostly unsuccessful because of their reproductive strategy. M. ehrenbergiiare hermaphroditic flatworms that can produce viviparous offspring (termed S eggs) and/or diapausing eggs (termed D eggs) and they follow either one of two reproductive patterns: (1) they first form S eggs and following the delivery of these eggs produce D eggs, or (2) they only produce D eggs. When only D eggs are formed, which is common under laboratory conditions, the stocks die out until the diapausing eggs hatch, which is irregular and creates unpredictable wait times. As a result, to maintain M. ehrenbergii stocks in order to study their spermatocytes, we studied various factors that might influence egg type production. We have found that feeding them daily and keeping them at 25°C favours S egg production. Currently, our cultures have reached the 45th generation. In this article we describe our rearing and dissection methods and describe experiments which led to our present rearing methods.en_US
dc.identifier.citationCell Biol Int. 2013 Oct;37(10):1089-105.en_US
dc.identifier.issn1065-6995
dc.identifier.urihttps://doi.org/10.1002/cbin.10129en_US
dc.identifier.urihttp://hdl.handle.net/10315/38105
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsWiley This is the peer reviewed version of the following article: [Cell Biol Int. 2013 Oct;37(10)], which has been published in final form at [https://doi.org/10.1002/cbin.10129]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.rightsAttribution-NoDerivatives 4.0 International*
dc.rights.articlehttps://pubmed.ncbi.nlm.nih.gov/23686667/en_US
dc.rights.journalhttps://www.ncbi.nlm.nih.gov/en_US
dc.rights.publisherhttps://onlinelibrary.wiley.comen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/*
dc.subjectcell divisionen_US
dc.subjectegg typeen_US
dc.subjectmeiosisen_US
dc.subjectspermatocytesen_US
dc.subjectmesostomaen_US
dc.titleMethods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg typesen_US
dc.title.alternativeRearing Mesostoma in the lab for cell biologyen_US
dc.typeArticleen_US

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