Biochemical Studies of EA22, A Protein Involved in The Bacteriophage Lysogenic-Lytic Decision and the Virulence of Clinically Relevant Serotypes of E. Coli

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dc.contributor.advisorDonaldson, Logan
dc.contributor.authorTong, Jinge
dc.date.accessioned2022-12-14T16:30:40Z
dc.date.available2022-12-14T16:30:40Z
dc.date.copyright2022-07-21
dc.date.issued2022-12-14
dc.date.updated2022-12-14T16:30:40Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractEnterohemorrhagic Escherichia coli (EHEC) infections are associated with short term gastrointestinal problems, but in some cases, it can lead to more life-threatening conditions. The pathogenicity of EHEC Is supported by an endogenous, dormant bacteriophage that persists in the bacterium as a lysogen until it is activated by a stress-related event. While the lysogenic-lytic development decision has been characterized for decades, there are still genes that are unknown in every way, from structure to function. These genes are found in a region between exo and xis in the l bacteriophage genome which may serve as a model for understanding other related phages in EHEC. This thesis focuses on the protein encoded by the ea22 gene, the largest conserved gene in the exo-xis region. The most significant finding of my research was the expression and characterization of several Ea22 proteins and protein fragments from l and two phages associated with EHEC. Structural differences in the C-terminal domains of these proteins may help explain the broad range of effects the Ea22 proteins mediate during infection.
dc.identifier.urihttp://hdl.handle.net/10315/40692
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsBiology
dc.subject.keywordsProtein structure
dc.subject.keywordsConformational study
dc.subject.keywordsexo-xis protein
dc.subject.keywordsBacteriophage
dc.subject.keywordsE.coli
dc.subject.keywordsLysogenic-lytic decision
dc.titleBiochemical Studies of EA22, A Protein Involved in The Bacteriophage Lysogenic-Lytic Decision and the Virulence of Clinically Relevant Serotypes of E. Coli
dc.typeElectronic Thesis or Dissertation

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