Fluorometry studies of aptamers that bind intrinsically fluorescent ligands: techniques, obstacles and optimizations
| dc.contributor.author | Shoara, Aron A | |
| dc.contributor.author | Johnson, Philip E | |
| dc.date.accessioned | 2022-10-14T14:44:07Z | |
| dc.date.available | 2022-10-14T14:44:07Z | |
| dc.date.issued | 2022-09-26 | |
| dc.description.abstract | Intrinsic fluorescence analysis is a sensitive technique to gain insight about aptamer-small molecule interactions.Employing fluorescence properties of the ligand, the binding affinities of the aptamer-ligand complex can be quantified in the nM to mM range with great accuracy and precision. Here, we present a detection method for aptamer-ligand binding analysis that is based on the inherent fluorescence of the ligand. Further, we discuss how to optimize and resolve some common experimental challenges. | en_US |
| dc.identifier.citation | Aptamers 6 (2022) 19-27 | en_US |
| dc.identifier.issn | 2514-3247 | |
| dc.identifier.uri | http://hdl.handle.net/10315/39762 | |
| dc.language.iso | en | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.article | http://japtamers.co.uk/fluorometry-studies-of-aptamers-that-bind-intrinsically-fluorescent-ligands-techniques-obstacles-and-optimizations/ | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.title | Fluorometry studies of aptamers that bind intrinsically fluorescent ligands: techniques, obstacles and optimizations | en_US |