Possible roles of actin and myosin during anaphase chromosome movements in locust spermatocytes

dc.contributor.authorForer, Arthur
dc.contributor.authorFabian, Lacramioara
dc.date.accessioned2021-02-22T18:59:50Z
dc.date.available2021-02-22T18:59:50Z
dc.date.issued2007-10
dc.description.abstractWe tested whether the mechanisms of chromosome movement during anaphase in locust [Locusta migratoria (L.)] spermatocytes might be similar to those described in crane-fly spermatocytes. Actin and myosin have been implicated in anaphase chromosome movements in crane-fly spermatocytes as indicated by effects of inhibitors and by localisations of actin and myosin in spindles. In this study we tested whether locust spermatocytes spindles also utilize actin and myosin and whether actin is involved in microtubule flux. Living locust spermatocytes were treated with inhibitors of actin (Latrunculin B and Cytochalasin D), an inhibitor of myosin (BDM), or inhibitors of myosin phosphorylation (Y-27632 and ML-7). We added drugs (individually) during anaphase. Actin inhibitors alter anaphase: chromosomes either completely stop moving, slow, or sometimes accelerate. The myosin inhibitor, BDM, also alters anaphase: in most cases, the chromosomes drastically slow or stop. ML-7, an inhibitor of MLCK, causes chromosomes to stop, slow, or sometimes accelerate, similar to actin inhibitors. Y27632, an inhibitor of Rho-kinase, drastically slows or stops anaphase chromosome movements. The effects of the drugs on anaphase movement are reversible: most of the half-bivalents resume movement at normal speed after these drugs are washed out. Actin and myosin were present in the spindles in locations consistent with their possible involvement in force production. Microtubule flux along kinetochore fibres is an actin-dependent process, since LatB removes completely or drastically reduces the gap in microtubule acetylation at the kinetochore. These results suggest that actin and myosin are involved in anaphase chromosome movements in locust spermatocytes.en_US
dc.identifier.citationProtoplasma 231, 201–213 (2007).en_US
dc.identifier.issn0033-183X
dc.identifier.urihttps://doi.org/10.1007/s00709-007-0262-yen_US
dc.identifier.urihttp://hdl.handle.net/10315/38110
dc.language.isoenen_US
dc.publisherSpringer Linken_US
dc.rightsSpringer This is a post-peer-review, pre-copyedit version of an article published in Protoplasma 231, 201–213 (2007). The final authenticated version is available online at: https://doi.org/10.1007/s00709-007-0262-y. More information on Springer Nature terms of reuse for archived author accepted manuscripts (AAMs) of subscription articles can be found at https://www.springer.com/gp/open-access/publication-policies/aam-terms-of-use.en_US
dc.rightsAttribution-NoDerivatives 4.0 International*
dc.rights.articlehttps://link.springer.com/article/10.1007%2Fs00709-007-0262-yen_US
dc.rights.journalhttps://www.springer.com/journal/709/en_US
dc.rights.publisherhttps://link.springer.com/en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/*
dc.subjectchromosome movementen_US
dc.subjectmitosisen_US
dc.subjectactinen_US
dc.subjectmyosinen_US
dc.subjectlatrunculin Ben_US
dc.subject2,3-butanedione monoximeen_US
dc.subjectmyosin phosphorylation inhibitoren_US
dc.titlePossible roles of actin and myosin during anaphase chromosome movements in locust spermatocytesen_US
dc.title.alternativeChromosome movements in locust spermatocytesen_US
dc.typeArticleen_US

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