A Comparative Study Performed on Two Ambient Ionization Techniques: Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) and Easy Ambient Sonic Spray Ionization Mass Spectrometry (EASI-MS)

Simple item page
dc.contributor.advisorIfa, Demian R.
dc.creatorHamid, Tanam Sanjana
dc.date.accessioned2015-08-28T15:08:44Z
dc.date.available2015-08-28T15:08:44Z
dc.date.copyright2014-11-21
dc.date.issued2015-08-28
dc.date.updated2015-08-28T15:08:44Z
dc.degree.disciplineChemistry
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMain project of my master’s thesis involved comparison between two sister methods: desorption electrospray ionization mass spectrometry (DESI−MS) and easy ambient ionization mass spectrometry (EASI−MS). These methods are compared in terms of spatial resolution, limits of detection and imaging capabilities. Their applicability in forensic science was further explored by comparing the MS/MS imaging capabilities. Under the same experimental conditions, we found that both DESI and EASI−MS have similar spray spot size resulting in similar spatial resolution. For the limits of detection (LOD) experiment, the LOD of DESI−MS was found to be lower than EASI−MS by an order of magnitude. When we compared the mass spectra and the chemical images of DESI and EASI, we found that both methods produce similar chemical specificity. When the spectra were carefully inspected, a reduction in signal intensity was observed for EASI−MS compared to DESI−MS demonstrating that the sensitivity of DESI−MS is higher than EASI−MS. Other projects involved characterization of zebra fish tissue system made up of bile salts, phospholipids and fatty acids. By mapping the spatial distribution of these ions, 2D chemical images of organs such as the stomach, the nervous system and the whole body zebra fish were created. I also studied if matrix can impact ionization of proteins by DESI−MS. The aim was to improve the poor desorption of proteins from the surface of DESI−MS. We concluded that the matrix neither increase nor decreases the ionization efficiency of proteins by DESI−MS.
dc.identifier.urihttp://hdl.handle.net/10315/29957
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectChemistry
dc.subjectAnalytical chemistry
dc.subject.keywordsMass spectrometry
dc.subject.keywordsDESI−MS
dc.subject.keywordsZebra fish
dc.subject.keywordsSpatial resolution
dc.subject.keywordsImaging mass spectrometry
dc.subject.keywordsAmbient Ionization mass spectrometry
dc.subject.keywordsMatrix effects on protein analysis
dc.titleA Comparative Study Performed on Two Ambient Ionization Techniques: Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) and Easy Ambient Sonic Spray Ionization Mass Spectrometry (EASI-MS)
dc.typeElectronic Thesis or Dissertationen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Hamid_Tanam_S_2014_Masters.pdf
Size:
2.03 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.83 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.38 KB
Format:
Plain Text
Description:
Download

Collections

Chemistry

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.