The Effects of Nutrient Availability and O-GlcNAcylation on Pericyte Differentiation Fate

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dc.contributor.advisorHaas, Tara
dc.contributor.authorAzimikhiabani, Barbod
dc.date.accessioned2023-03-28T21:25:42Z
dc.date.available2023-03-28T21:25:42Z
dc.date.copyright2023-01-27
dc.date.issued2023-03-28
dc.date.updated2023-03-28T21:25:41Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractPericytes are mural cells on the surface of capillaries, which may have the capacity to differentiate into various lineages. However, the factors that could determine their differentiation fate remain to be determined. I hypothesized that nutrient availability, O-GlcNAcylation status, and metabolic stress could alter pericyte phenotype. In my experiments, I used mesenchymal cells from the skeletal muscle of C57B16/J mice as a model, due to the similarity in the characteristics of mesenchymal cells and pericytes. My data showed that Zfp423 protein levels are higher when exposed to normal glucose levels (5 mM), compared to high glucose levels (25 mM), which leads to higher fat droplet formation in mesenchymal cells. However, my data did not support the influence of O-GlcNAcylation on adipogenesis. Lastly, I observed that Transforming growth factor-β1 could potentially induce myocyte-looking cell formation. This finding provides an insight into how nutrient availability could influence the differentiation potential of pericytes.
dc.identifier.urihttp://hdl.handle.net/10315/41060
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subjectKinesiology
dc.subjectCellular biology
dc.subject.keywordsPericyte
dc.subject.keywordsDifferentiation fate
dc.subject.keywordsMesenchymal cells
dc.subject.keywordsStem cells
dc.subject.keywordsNutrient availability
dc.subject.keywordsO- GlcNAcylation
dc.subject.keywordsPhenotype change
dc.subject.keywordsCapillaries
dc.subject.keywordsZfp423
dc.subject.keywordsAdipogenesis
dc.subject.keywordsFibrogenesis
dc.subject.keywordsMultipotency
dc.subject.keywordsMetabolic stress
dc.subject.keywordsGlucose levels
dc.titleThe Effects of Nutrient Availability and O-GlcNAcylation on Pericyte Differentiation Fate
dc.typeElectronic Thesis or Dissertation

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