Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes

dc.contributor.authorForer, Arthur
dc.contributor.authorSpurck, Tim
dc.contributor.authorPickett-Heaps, Jeremy
dc.date.accessioned2021-02-22T20:57:12Z
dc.date.available2021-02-22T20:57:12Z
dc.date.issued2007-12
dc.description.abstractWe used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphasecrane-fly spermatocytes; then we followed the growth of the “kinetochore stubs”, the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subunits at the kinetochore, and thus elongation is related to flux of tubulin in the kinetochore microtubules. Stub elongation was blocked by cytochalasin D and latrunculin A, actin inhibitors, and by butanedione monoxime, a myosin inhibitor. We conclude that actin and myosin are involved in generating elongation and thus in producing flux of tubulin in kinetochore microtubules. We suggest that actin and myosin act in concert with a spindle matrix to propel kinetochore fibres poleward thereby causing stub elongation and generating anaphasechromosome movement in non-irradiated cells.en_US
dc.identifier.citationProtoplasma 232, 79–85 (2007).en_US
dc.identifier.issn0033-183X
dc.identifier.urihttps://doi.org/10.1007/s00709-007-0265-8en_US
dc.identifier.urihttp://hdl.handle.net/10315/38111
dc.language.isoenen_US
dc.publisherSpringer Linken_US
dc.rightsSpringer This is a post-peer-review, pre-copyedit version of an article published in Protoplasma. 2007;232(1-2):79-85.. The final authenticated version is available online at: https://doi.org/10.1007/s00709-007-0265-8 More information on Springer Nature terms of reuse for archived author accepted manuscripts (AAMs) of subscription articles can be found at https://www.springer.com/gp/open-access/publication-policies/aam-terms-of-use.en_US
dc.rightsAttribution-NoDerivatives 4.0 International*
dc.rights.articlehttps://pubmed.ncbi.nlm.nih.gov/18094930/en_US
dc.rights.journalhttps://www.ncbi.nlm.nih.gov/en_US
dc.rights.publisherhttps://link.springer.com/en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/*
dc.subjectchromosome movementen_US
dc.subjectspindle functionen_US
dc.subjectcrane-fly spermatocytesen_US
dc.subjectbutanedionemonoximeen_US
dc.subjectcytochalasinen_US
dc.subjectlatrunculinen_US
dc.subjectUV microbeamen_US
dc.titleActin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytesen_US
dc.title.alternativeActin, myosin and tubulin fluxen_US
dc.typeArticleen_US

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