Analysis of Different Design Approaches to Favor the Growth of Polyhydroxybutyrate (PHB) Producers in a Methane Utilizing Mixed Culture Community

Simple item page
dc.contributor.advisorAudette, Gerald F.
dc.contributor.advisorEldyasti, Ahmed
dc.contributor.authorSalem, Rana Khaled Moselhy Ahmed
dc.date.accessioned2020-11-13T13:43:42Z
dc.date.available2020-11-13T13:43:42Z
dc.date.copyright2020-06
dc.date.issued2020-11-13
dc.date.updated2020-11-13T13:43:42Z
dc.degree.disciplineCivil Engineering
dc.degree.levelMaster's
dc.degree.nameMASc - Master of Applied Science
dc.description.abstractCurrently, petroleum-based plastic disposal and its subsequent accumulation in the environment have become a major concern in recent years. Hence, the industrialization of bioplastics, PHA biopolymer has gained broad interest in recent years. This thesis offers an efficient approach for maximizing PHB polymer production in methanotrophic bacteria through using the methane gas produced in the anaerobic digestion process in wastewater treatment plants (WWTPs). This is achieved through offering an innovative selection approach for PHB producers including type II methanotrophs whilst enabling other PHB accumulators to grow simultaneously. PHB accumulation reached around 60% accumulation in ammonia mineral salt (AMS) media and 55% in nitrate mineral salt (NMS), which is higher than what has been reached in the literature for both nitrogen sources. Furthermore, characterization of the PHB polymer with Sudan Black B dye and NMR. Lastly, an optimization of the operational parameters; using central composite design (CCD) have been performed.
dc.identifier.urihttp://hdl.handle.net/10315/37860
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPolymer chemistry
dc.subject.keywordsWastewater
dc.subject.keywordsPolyhydroxybutyrate
dc.subject.keywordsBioplastic
dc.subject.keywordsMethanotrophs
dc.subject.keywordsNMR
dc.subject.keywordsOptimization
dc.subject.keywordsCentral composite design
dc.subject.keywordsSudan black
dc.titleAnalysis of Different Design Approaches to Favor the Growth of Polyhydroxybutyrate (PHB) Producers in a Methane Utilizing Mixed Culture Community
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Salem_Rana_K_2020_Masters.pdf
Size:
2.74 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.83 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.36 KB
Format:
Plain Text
Description:
Download

Collections

Civil Engineering

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.