Rapid characterization of folding and binding interactions with thermolabile ligands by DSC
| dc.contributor.author | Harkness V, Robert W | |
| dc.contributor.author | Slavkovic, Sladjana | |
| dc.contributor.author | Johnson, Philip E. | |
| dc.contributor.author | Mittermaier, Anthony K. | |
| dc.date.accessioned | 2023-05-10T17:13:55Z | |
| dc.date.available | 2023-05-10T17:13:55Z | |
| dc.date.issued | 2016-10-23 | |
| dc.description.abstract | Differential scanning calorimetry (DSC) is a powerful technique for measuring tight biomolecular interactions. However, many pharma- ceutically relevant ligands are chemically unstable at the high temperatures used in DSC analyses. Thus, measuring binding inter- actions is challenging because the concentrations of ligands and thermally-converted products are constantly changing within the calorimeter cell. Using experimental data for two DNA aptamers that bind to the thermolabile ligand cocaine, we present a new global fitting analysis that yields the complete set of folding and binding parameters for the initial and final forms of the ligand from a pair of DSC experiments, while accounting for the thermal conversion. Furthermore, we show that the rate constant for thermolabile ligand conversion may be obtained with only one additional DSC dataset. | en_US |
| dc.identifier.citation | Chem. Commun. 52 (2016): 13471--13474 | en_US |
| dc.identifier.uri | https://doi.org/10.1039/c6cc05576a | en_US |
| dc.identifier.uri | http://hdl.handle.net/10315/41140 | |
| dc.language.iso | en | en_US |
| dc.publisher | The Royal Society of Chemistry | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.title | Rapid characterization of folding and binding interactions with thermolabile ligands by DSC | en_US |
| dc.type | Article | en_US |