Shoara, Aron AJohnson, Philip E2022-10-142022-10-142022-09-26Aptamers 6 (2022) 19-272514-3247http://hdl.handle.net/10315/39762Intrinsic fluorescence analysis is a sensitive technique to gain insight about aptamer-small molecule interactions.Employing fluorescence properties of the ligand, the binding affinities of the aptamer-ligand complex can be quantified in the nM to mM range with great accuracy and precision. Here, we present a detection method for aptamer-ligand binding analysis that is based on the inherent fluorescence of the ligand. Further, we discuss how to optimize and resolve some common experimental challenges.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://japtamers.co.uk/fluorometry-studies-of-aptamers-that-bind-intrinsically-fluorescent-ligands-techniques-obstacles-and-optimizations/