Abdalla, MarwaLento, CristinaJiang, GuifengPower, AustinWilson, Derek2023-06-082023-06-082023-05-12Marwa ElSabaawy, Cristina Lento and Guifeng Jiang et al. Proline/Alanine Tails as Ionization Enhancement Tags in Native Mass Spectrometry. ScienceOpen Posters. 2023. DOI: 10.14293/P2199-8442.1.SOP-.PYK0FB.v1https://doi.org/10.14293/P2199-8442.1.SOP-.PYK0FB.v1http://hdl.handle.net/10315/41196This study aimed to study the impact of Proline/Alanine tag on l-asparaginase protein dynamics. Our current analysis demonstrates that the PA tail fragment did not change the core protein dynamics using TRESI HDX MS to provide identical stability with untagged l-asparaginase. Novel Aspects In this work, we propose PASylation ® as a new tool for ionization enhancement through chain ejection.enPoster